The Development and Recent Improvements of in Vitro Nucleic Acid Amplification Technology

Rapid nucleic acid amplification in vitro is a revolutionary technology developed since 1983.It has been applied widely in modern agriculture,medical and food science etc..Such technology has provided a great tool for gene manipulation and functional genomics.In vitro nucleic acid amplification is usually carried out by a thermal stable enzyme,such as Taq polymerase,and requires thermal cycle equipment.The invention of isothermal amplification has improved the traditional PCR method and in some way simplified the reaction procedure.Recombinase-aid amplification(RAA) was introduced.RAA is the latest isothermal amplification technology which may be carried out under the room temperatures.The distinctive merit of RAA is mimicking in vivo T4-phage DNA amplification complex and the whole reaction is super fast and specific.Moreover,it can also be used for digital real time quantitative analysis.