Effects of pyloric local CGRP and NO on bile reflux in rat stomach with stress ulcer

AIM: To investigate the effects of pyloric local CGRP and NO on bile reflux in rat stomach with stress ulcer. METHODS: Rats stress ulcer model was established by cold water soaking. Sixty-five rats were divided into three groups: normal control group, the stress group and the antagonist group. The gastric ulcer index, pH and bile acid of gastric juice were measured before and after stress. The pyloric local CGRP was measured by a radioimmu- noassay detection kit. The pyloric local nitric oxide was measured by a biochemic detection kit. RESULTS: The pyloric local nitric oxide reached the climax at 1 hour after stress. The bile acid and pH of gastric juice reached the maximum at 2 hours after stress. The ulcer index did it at 4 hours after stress. But the pyloric local CGRP decreased to the minimum at 4 hours after stress. The bile acid and ulcer index in L-NAME group decreased significantly compared with those of h-CGRP8-37 group. However, the bile acid of hCGRP837 group was less than that of antagonist control group, which was weaker than that in L-NAME group.