Enzymatic Synthesis of Pure Maltohexaose from α-Cyclodextrin

Maltohexaose (G6) is a maltodextrin consisted of six-glucose in α-1,6-glycosidic linkages and essential research tool in food, cosmetic and pharmaceutical. In previous study, we have proven that the possibility of large-scale continuous production of pure maltodextrins from cyclodextrins (CDs) using Pyrococcus furiosus thermostable amylase with immobilization in a packed-bed reactor (PBR). In this study, we used this PBR for G6 production from α-CD. After enzyme reaction, 1-decanol was added to G6 mixture as precipitating agent with a 1:1 ratio of 1-decanol to α-CD. In which, the unreacted α-CD was precipitated, then it was separated by centrifugation. Finally, the G6-product was obtained in homogeneous form and high-purity (95 %). Through this study, we successfully demonstrated the enzymatic synthesis method to produce pure G6 from α-CD.