One-step electrodeposition of poly(m-aminobenzoic acid) membrane decorated with peptide for antifouling biosensing of Immunoglobulin E

Abstract m-Aminobenzoic acid (ABA) is one popular derivative of highly conductive monomer of aniline, which contains a carboxyl (-COOH) group in its skeleton that is beneficial to various bio-interface and bio-analysis. Hence, Poly(m -aminobenzoic acid) (PABA) membrane was firstly electrochemical deposited onto bare electrode surface using a straightforward cyclic voltammetry (CV) method. PABA membrane exhibited excellent electrochemical stability and apparent wrinkle morphology that could effectively enhance response signal and immensely increase specific surface area of electrode. Next, PABA membrane was decorated with well-designed hairpin aptamer and preferred antifouling peptide in sequence to construct a two-layer architectural bio-interface that could present both sensitive target recognition capability and excellent antifouling ability. Benefiting from the electrodeposited PABA membrane to enhance electrochemical response signal, the developed biosensor performed excellent sensitivity toward target protein, meanwhile, associated with good selectivity and reproducibility attributing to the favored peptide that was able to decline nonspecific protein adsorption and improve target recognition.