Presence of GLUT4 and SGLT1 in ductal cells of normal and streptozotocin rat salivary glands

MATERIAL AND METHODS Staining included avidin-biotin-peroxidase (Vector Labs, Belgium) and diaminobenzidine (Dako, Belgium), with hematoxylin counterstaining for the DAB technique; and fluorescein isothyocyanate (FITC, Jackson) for immunofluorescence. Anti-Glut4 antibody (Millipore 07-1404, 1/1000) and anti-SGLT1 antibody (Millipore 07-1417, 1/1000) were applied on the fixed normal and streptozotocin rat salivary glands sections. Total RNA from salivary glands was extracted and subjected to mRNA quantification using specific primers for SGLT1 and Glut4 and SYBR Green on a LightCycler 480.