Study on the Extraction of Huperzine A from Huperzia serrata by Enzymatic Method

[Objective] To screen the optimum extraction technology of huperzine A from Huperzia serrata by enzymatic method.[Method] Using cellulase to hydrolyze the cytoderm,orthogonal test was carried out to optimize the extraction technology of huperzine A from Huperzia serrata by enzymatic method.[Result] The optimum extraction technology of huperzine A from Huperzia serrata by enzymatic method was a combination of 0.125 g cellulase,4.5 pH,60 ℃ enzymolysis temperature and 2.5 h enzymolysis time,under which the extraction rate of huperzine A could reach 0.589‰.Compared with the acidsoak method,the extraction rate of huperzine A by enzymatic method increased by 40.3%.Besides,hydrolysis temperature was the biggest influence factor to the extraction of huperzine A from Huperzia serrata by enzymatic method.[Conclusion] This method was simple,convenient and efficient,which could be used for extracting huperzine A from Huperzia serrata.