Expressão de metaloproteinases de matriz e de seus inibidores teciduais em carcinomas basocelulares Expression of matrix metalloproteinasis and their tissue inhibitors in basal cell carcinoma

3 Introducao: Aproximadamente 80% das neoplasias malignas de pele nao-melanomas sao carcinomas basocelulares (CBC). Apesar das raras metastases, esses tumores sao localmente agressivos. As metaloproteinases de matriz (MMPs), especialmente as MMP-2 e 9, sao importantes no processo de invasao. Em contrapartida, os inibidores teciduais das MMPs (TIMPs) tem como principal funcao a inibicao dessas enzimas. Objetivo: Investigar a associacao de variaveis clinicopatologicas de pacientes portadores de CBC com a expressao de MMP-2, MMP-9, TIMP-1 e TIMP-2. Material e metodos: Foram selecionados 31 casos de CBC, sendo entao obtidos, retrospectivamente, os dados referentes a idade, sexo e tamanho da lesao. Cortes histologicos das lesoes foram submetidos a reacao imuno-histoquimica pela tecnica estreptavidina-biotina-peroxidase para deteccao dos antigenos de interesse. Indices de imunomarcacao foram construidos e comparados com os dados previamente obtidos. Resultados: Observou-se correlacao significativa entre idade e tamanho da lesao ( R = 0,532; p = 0,008). Nao foram observadas correlacoes significativas entre as outras variaveis e a expressao imuno-histoquimica dos antigenos de interesse. Conclusao: A expressao das metaloproteinases e de seus inibidores teciduais nao parece ser influenciada pelos parâmetros investigados. Estudos adicionais sao necessarios para melhor compreensao de sua associacao com o comportamento biologico do CBC. abstract resumo Introduction: Approximately 80% of non-melanoma skin neoplasias are basal cell carcinomas (BCC). Although metastasis is rare, BBC carcinomas are locally aggressive tumors. Matrix metalloproteinases (MMPs), mainly MMP-2 and MMP-9, play an important role on the invasion process. On the other hand, tissue inhibitors of MMPs (TIMPs) have the main function of inhibiting these enzymes. Objective: To investigate the association of clinical-pathological variables of BCC patients with the expression of MMP-2 and MMP-9, TIMP-1 and TIMP-2. Methods: Thirty-one BCC cases were selected. Gender, age of the patients and size of the lesions were obtained retrospectively. Histological cuts of the lesions were exposed to immunohistochemistry reaction by use of the streptavidine-biotin peroxidase technique in order to detect antigens. Immunomarking parameters were established and compared with previous data. Results: A significant correlation between age and size of the lesion was observed (R = 0.532; p = 0.008). No significant correlations between other variables and immunohistochemical expression of antigens were observed. Conclusion: The expression of MMPs and TIMPs does not seem to be influenced by the parameters investigated in this work. Additional studies should be made to better understand its association with the biological behavior of basal cell carcinomas. unitermos Carcinoma basocelular Metaloproteinase 2 Metaloproteinase 9 Inibidores teciduais de metaloproteinases de matriz 1 Inibidor tecidual de metaloproteinase de matriz 2 Imuno-histoquimica