Digital image colorimetric analysis for evaluating lipid oxidation in oils and its emulsion

Abstract Optical analytical images were captured by a digital camera, and lipid peroxidation was monitored using colorimetric determination. The Image-J method was shown to have comparable accuracy to the conventional titration (American Oil Chemists’ Society) and UV–Vis spectroscopic methods. Over the PV range 3–14 meq.O 2 /kg, a good correlation (R 2  = 0.9836) was found between the proposed method and UV–Vis method with an SD of ±0.0339 meq.O 2 /kg. The range and accuracy of detection were determined from the changes in the Image-J intensity values. At equivalent PV, the limit of detection was 0.175 meq.O 2 /kg and the limit of quantitation was 0.35 meq.O 2 /kg. The proposed Image-J application can be used to develop fast and portable devices for monitoring peroxide values. These have potential applications in quality control for small-scale farmers and small and medium enterprises.