[Screening of polypeptides interacting with Rel homology domain of NF-kappaB p50 subunit].

AIM: To obtain the polypeptides interacting with NF-κB p50 Rel homology domain(RHD) by yeast two-hybrid technique.METHODS: Using NF-κB p50 RHD as bait,the polypeptides interacting with NF-κB p50 RHD were screened from a 16-peptide cDNA library by yeast two-hybrid technique.The false positive clones were screened out but the positive clone were identifyied by β-GAL assay,yeast mating,and one to one yeast two-hybrid method.RESULTS: 8 positive clones were obtained.The gene sequences of the eight polypeptides were different from each other and their homologous proteins were not found in GenBank.CONCLUSION: 8 novel polypeptides interacting with NF-κB p50 RHD were obtained,but their functions need to be validated in further experiments.