SgRNA (small guide ribonucleic acid) pair for specifically identifying porcine H11 locus, and coding DNA (deoxyribonucleic acid) and application thereof

The invention provides an sgRNA (small guide ribonucleic acid) pair for specifically identifying porcine H11 locus, and a coding DNA (deoxyribonucleic acid) and application thereof. The sgRNA pair is composed of sgRNA-F and sgRNA-R, wherein the nucleotide sequence of the sgRNA-L is 1) or 2): 1) nucleotide sequence disclosed as Sequence 1 in the sequence table, and 2) nucleotide sequence with the same functions as the nucleotide sequence disclosed as 1) subjected to substitution and/or deletion and/or addition of one or more bases on the nucleotide sequence disclosed as 1); and the nucleotide sequence of the sgRNA-R is 3) or 4): 3) nucleotide sequence disclosed as Sequence 2 in the sequence table, and 4) nucleotide sequence with the same functions as the nucleotide sequence disclosed as 3) subjected to substitution and/or deletion and/or addition of one or more bases on the nucleotide sequence disclosed as 3). The sgRNA pair can greatly increase the fixed point insertion efficiency of the exogenous gene, and provides a stable platform for preparation of transgenic pigs.