Verification of cucumber-sour cucumber introgression lines and screening resistant to gummy stem blight.

[Objectives]The aim of this study is to validate the introgression lines derived from the cross of cucumber and sour cucumber,and obtain materials resistant to gummy stem blight from introgression lines. [Methods]Seedlings of 86 introgression lines descended from cucumber-sour cucumber hybrids were subjected to artificial inoculation under greenhouse condition to screen for materials resistant to gummy stem blight. And agronomical traits were observed and analyzed as well. SSR specific to sour cucumber parent were used for confirming the introgression lines. [Results]The results showed that the introgression lines carryied some sour cucumber morphological traits,such as black spine,short internode and multi-branching. SSR analysis indicated that the introgression lines could amplify sour cucumber specific bands which confirmed these introgression lines containing chromosomal fragments from sour cucumber. Based on these analyses,8 resistant materials(CSIL2,CSIL41,CSIL49,CSIL52,CSIL57,CSIL58,CSIL59,CSIL72) were selected from the cucumber-sour cucumber introgression lines,accounting for 9% of the tested materials. [Conclusions]Introgression lines descended from wide hybridization can be used to broaden the genetic base of crops,and the 8 resistant materials selected from cucumber introgression lines provide resistant resource for genetic research of gummy stem blight and breeding practices.