Dynamized follicle-stimulating hormone affects the development of ovine preantral follicles cultured in vitro

Objective To evaluate the effect of dynamized follicle-stimulating hormone (FSH) on the survival, activation and growth of ovine preantral follicles (PFs) in vitro . Methods Ovarian fragments were cultured for 1 or 7 days in alpha minimum essential medium (α-MEM + ) control in the absence or presence of alcohol (Al control) or FSH (6cH, 12cH and 30cH) added at intervals of 24 or 48 h. The ovarian fragments were processed, coded and analyzed by a blinded observer by classical histology (CH), fluorescence microscopy (FM) and transmission electron microscopy (TEM). Results After 7 days of culture, the group which to which FSH 6cH was added at 24 h intervals showed better rates of follicle survival and activation compared to α-MEM + control or Al control ( p + control ( p Conclusions FSH 6cH (24 h) treatment maintained the viability, and promoted the activation and in vitro growth of ovine PFs.