转hMCP／hCD59双基因小鼠的构建、传代及功能测试

Objective Comparing protective effect on the cell from one CRP or two CRPs. Method Double gene mouse was founded by mixed injection. Mixed human MCP and CD59 gene was injected into pronuclear of 521 mice zygote. Results In all of the newborn mouse, there are 14 with CD59 gene, 12 with MCP gene, 7 with double gene. Total transgene rate was 5.0 percent. Five double gene founders mated with nontransgene mouse, which their baby had 14 with MCP gene, 16 with CD59 gene, 10 with double gene corresponded to 33.3 percent of baby mouse. The hearts in vitro were taken out to be perfused with fresh human blood plasma, the average heart beating time was 138±25 min in the hCD59/hMCP group. The time is significantly longer than that of other group (78±27 min in the hCD59 group, 43±21 min in the hMCP group, and 20±12 min in the nontransgenic group). Conclusion The organ of double gene mouse is better to resist immunity attacking than one gene mouse.