Effects of Fibronectin Fragments on DNA Transfection into Mammalian Cells by Electroporation

: Two kinds of cells, human epidermoid carcinoma A431 cells and simian kidney COS-7 cells, were transfected with the chloramphenicol acetyl transferase (CAT) gene by electroporation, and then cultivated on culture dishes coated with two different forms of recombinant fibronectin fragments consisting of cell-binding domain (C-274) or heparin-binding domain and CS1 region (H-296). In the case of A431 cells, H-296-coated dishes significantly increased the amount of expressed CAT and the adhesion of electroporated cells in comparison with non-coated dishes. C-274 was effective for COS-7 cells. Overall, these fibronectin fragments increased the recovery of the transfectants on A-431 cells and COS-7 cells, respectively.