Cyanogen Bromide Cleavage of Proteins on Blots and Subsequent Separation of the Fragments by Polyacrylamide Gel Electrophoresis Directly from those Blots

Since nucleic acid sequencing is now quick and accurate, the usual goal of protein sequencing is no longer to determine an entire primary structure. A partial sequence is sufficient for the design of the oligonucleotide probes, needed to obtain starting material for sequence determinations on the nucleic acid level.