REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF ARTESUNATE IN TABLET DOSAGE FORM

Artesunate is a new potent antimalarial drug and is a synthetic analogue of artemisinin. A simple, economical, fast, and precise reverse phase high performance liquid chromatographic method has been developed for the determination of Artesunate in tablet dosage form. The present method was developed on C-18 Hypersil (5 micron 25 cm× 4.6 mm) column, in isocratic mode with mobile phase acetonitrile: methanol (70: 30 v/v) at a constant flow rate of 1.0 ml/min and the detection wavelength was 211 nm. Proposed method was validated for precision, accuracy, linearity range, recovery, robustness and ruggedness. The developed method can be used for assay of Artesunate in pharmaceutical preparations.