Hepatoprotective reactivity of a copper-di-Schiffbase active centre analogue of Cu2Zn2 superoxide dismutase

Abstract 1. 1. The anti-inflammatory and hepatoprotective efficacy of CuPu(Py) 2 ({[ N , N ′-bis(2-pyridyl-methylene)-1,4-butanediamine]( N , N ′, N ″, N ′″)}-Cu 2+ ), a serum-stable, copper-di-Schiffbase active centre analogue of Cu 2 Zn 2 superoxide dismutase was tested in male NMNR mice suffering from endotoxin/galactosamine-induced hepatitis. 2. 2. Parameters including the activities of serum transaminases and sorbitol-dehydrogenase as well as the levels of reactive oxygen and nitrogen intermediates which were used to quantify the disease activity. 3. 3. A dose-dependent inhibition of hepatic enzyme release was noted in the presence of 0.1–10 mg/kg of CuPu(Py) 2 . 4. 4. The release of transaminases from damaged liver cells was reduced by 68% and paralleled the reduction of serum levels of nitric oxides. 5. 5. Elevated levels of reactive oxygen species were normalized to those healthy controls. 6. 6. The copper-free apochelate Pu(Py) 2 , which is unable to dismutate superoxide, did not display any anti-inflammatory reactivity.