[Immunological killing effect of recombicant adenovirus vector rAD-mTERT-m4-1BBL on mouse hepatoma cell line Hepa1-6 cells co-cultured with T lymphocytes].

Objective To study the immunological suppressing effect of recombinant adenovirus vector rAD-mTERT promotor-m4-1BBL （rAD-mTERT）on mouse hcpatoma cell line Hepal-6 cells in coculture with T lymphocytes. Methods Adding recombinant adenovirus rAD, rAD-CMV-m4-1BBL （rADCMV ） and rAD-mTERT to Hepal-6 and L929 cells, respectively, to observe the effect of these adenoviruses on growth and apoptosis of these cells in co-culture with T lymphocytes. Results Adding adenovirus significantly suppressed the growth and slightly increased apeptosis of the two types of cells （ P 〈 0.05 ）. rAD-mTERT promotor-m4-1BBL showed only pro-apoptotic effect on Hepal-6 cells. When co-cultured with T lymphocytes, rAD-CMV-m4-1BBL showed promoting effect on apoptosis of the cells. Compared with that of T cells pre-co-culture, CD4^＋ and CD^8＋ T cells were proliferated, and the ratio of CD4/CD8 was significantly reduced （from 1.27 to 1.08 ）. Conclusion Adding the recombinant adenoviruses only suppresses the cell growth, but not promotes their apoptosis. In co-culture with T lymphocytes, recombinant adenovirus vector rAD-mTERT promotor-m4-1 BBL can targetingly suppress the growth and induce apoptosis of Hepal-6 cells. The apoptosis is induced through the immunological killing effect of T lymphocytes. Key words: Telomerase reverse transcriptase;  Recombinant adenovirus vector;  Hepal-6 cells, mouse hepatoma cell line ;  T-lymphocytes ;  Immunity, cellular