Carbonaceous sorbents alongside an optimized magnetic solid phase extraction (MSPE) towards enrichment of crude Paclitaxel extracts from callus cultures of Taxus baccata

Abstract Here, a number of synthesized and commercial nano-sorbents including graphene (G), multi-wall carbon nanotube (MWCNT), graphene oxide (GO), reduced graphene oxide (rGO) and magnetic nanoparticles anchored on GO (Fe 3 O 4 NPs@GO) followed by graphite (Gt) powder were employed for paclitaxel pre-purification from callus culture-derived extract of Taxus baccata . Based on our preliminary work, rGO and Fe 3 O 4 NPs@GO exhibited the maximum potential, not only to remove impurities, but also to ameliorate taxol purity. According to the results of response surface methodology (RSM) developed for the superior nano-sorbent (i.e., Fe 3 O 4 NPs@GO), for both proposed quadratic polynomial models, statistically substantial relationships obtained between the actual and predicted values (‎p  3 O 4 NPs@GO nano-sorbent and RSM can be recommended as a simple, cost-effective and fast route for sorbent-assistant pre-purification investigations of paclitaxel.