Simple and Sensitive Detection of Hydrazine in Industrial Wastewater Using High-Performance Liquid Chromatography with Fluorescence Detector after Anthracene-2,3-dicarbaldehyde Derivatization

A simple and sensitive high-performance liquid chromatographic (HPLC) method was developed to determine the amount of hydrazine in industrial wastewater after derivatization with anthracene-2,3-dicarbaldehyde. The best reaction conditions (a reagent dosage of 200 mg/L, a pH of 11, and reaction for 1 min at room temperature) were established by the variation of parameters. The formed derivative was injected into the HPLC without further extraction or purification procedures. Under the established conditions, the limits of detection and the limits of quantification were 0.02 µg/L and 0.05 µg/L, respectively, and the inter-day relative standard deviation was less than 9% at concentrations of 0.1 and 0.5 µg/L. The calibration curve showed good linearity with r2 = 0.9999. The method was successfully applied to the determination of hydrazine in various kinds of industrial wastewater.