Cloning and expression analysis by real-time PCR of 4-coumarate: coenzyme A ligase (4CL) gene in Galega orientalis.

Galega orientalis L.was a pasture species which was introduced to China at the end of the twentieth century.The 4CL gene was cloned in Galega orientalis to explore its gene resources.The influence of 4CL gene on the stress-resistance in Galega orientalis was well studied.All of the research formed the basis for the implantation and application of Galega orientalis in China in future.With RACE and RT-PCR methods,the full length cDNA,which encode 4-coumarate: coenzyme A ligase,was cloned from Galega orientalis.The 1,916-bp full length Go4CL had a 1,653-bp ORF,which encodes 510 amino acids residues.The results from Real-Time PCR indicated that the expression of 4CL gene was the strongest in the root of Galega orientalis,and the least in leaves.Many abiotic stress treatments,such as PEG,ABA,MeJA and SA,all up regulated the expression of Go4CL gene,which indicated that 4CL may play a role in the biotic and abiotic stress-resistance regulation pathway in plants.