A multi-location peak parking approach for calculation of second dimensional retention indices for improved volatile compound identification with cryogen-free comprehensive heart-cut two-dimensional gas chromatography

Comprehensive heart-cut multidimensional gas chromatography (CH/C MDGC) without a cryogenic trapping device was developed with an established approach for calculation of first and second dimensional retention indices (1I and 2I) for improved compound identification. A first dimensional (1D) DB-1MS column (60 m) and a second dimensional (2D) DB-WAX column (60 m) were applied with a Deans switch (DS) using a constant H/C window of 0.2 min and a periodic multiple heartcut strategy comprising 225H/C throughout the CH/C. 1I was calculated based on comparison of the middle of the heartcut time with the alkane retention times on the 1D column. A multi-location peak parking approach using sixteen sets of automated injections of alkane references was also established with the least square curve fitting method for construction of the alkane isovolatility curves which were applied for 2I calculation. The untargeted compound analysis of a perfume sample was then performed according to comparison with the libraries of mass spectra, 1I and 2I. The CH/C MDGC system with a 25 h analysis time showed a peak capacity (nc) of 9198 and 128 separated peaks with 71 compounds successfully identified according to MS, 1I and 2I library match under the established error approximation criteria. Furthermore, relationship between the analysis time and number of separated peaks was proposed based on the set of 84 identifiable compounds. With the compensation of lower separation performance and greater I errors, the analysis time could be reduced by applying a 2.5 min H/C window with a total analysis time of 2 h and nc of 1134.