Toxicity screening of leaf extracts of Datura arborea Linn. (angel's trumpet) and Allamanda cathartica Linn. (yellow bell) using brine shrimp assay.

The toxicity of Datura arborea and Allamanda cathartica was studied using Brine Shrimp Assay. This experiment was a randomized controlled trial experiment which uses plant leaf extracts that were diluted in different concentrations (10%, 1%, 0.1% & 0.01%) and then were tested on the Brine shrimp. After 24 hours, the mortality of the brine shrimp for each concentration was accounted for. Each treatment had three trials. The leaf plant extracts of D. arborea and A. cathartica were both determined to be toxic. The highest concentration that affected the mortality of the brine shrimp from both plants was the 10% concentration. The lethal concentrations (LC50) of the two plants were determined in order to figure out at what point does the concentration become poisonous. The LC50 of D. arborea was 6.92%. The LC50 of A. cathartica was 5.27%. A. cathartica is more potent in terms of lethal concentration than D. arborea. The potency of A. cathartica is due to the allamandin toxin found in the plant. D. arborea’s toxicity depends on the combination of toxins of atropine, hyoscyamine, and scopolamine. The mortality of the brine shrimp was significantly affected by the toxicity of the D. arborea and A. cathartica.