THE EFFECT OF COPPER SULPHATE ON THE PRODUCTION OF FLAVONOIDS IN DIGITALIS LANATA CELL CULTURES

The paper investigated the effect of abiotic elicitor copper sulphate on the production of flavonoids in cell cultures of Digitalis lanata. Two cell lines were used (line 11 and line 13C-100) and two types of experiments were performed. In the first type of experiment the highest production of flavonoids was established, for both cell lines, for the strongest elicitor concentration (8µM). In the second type of experiment the maximal flavonoid production was induced for line 11 after 24 hour elicitation (over 10 times more compared with the control, from 0.624mg/g dry weight (d.w.) to 6mg/g d.w.), for the highest elicitor concentration(40µM). Rezumat In lucrarea de faţă am testat efectul sulfatului de cupru, un elicitor abiotic asupra producţiei de flavone in culturi celulare de Digitalis lanata. Au fost efectuate două tipuri de experimente, folosind două linii celulare de Digitalis lanata: linia 11 si linia 13C-100. Pentru primul tip de experiment, cele mai mari cantităţi de flavone se acumulează, pentru ambele linii celulare, la concentraţia cea mai mare a elicitorului (8µM). In ceea ce priveste al doilea tip de experiment, linia 11 sintetizează cantitatea cea mai mare de flavone (de aproximativ 10 ori mai mult comparativ cu martorul, de la 0,624mg/g s.u. (substanţă uscată) la 6mg/g s.u., după 24 ore de elicitare, folosind concentraţia cea mai mare de sulfat de cupru, 40µM.