Image processing system for particle distributions auto-analysis of in-line holograms

This presentation describes an on-line image analysis system for the automatic distribution analysis of holographic particles in 3D space. To obtain the 3D distribution parameters of particles, sequences of 2D cross-sectional retrieved images of the particle hologram are obtained using the in-line retrieval method, and the processing of the 2D retrieved images is discussed in this presentation. To segment the candidate particles, an entropy based automatic threshold selection method is adopted. In the process of out-of-focus particle removal, the radial intensity profile of the candidates in the original image and the clearness of the candidate neighboring areas in the Sobeled image are analyzed. Experimental results are presented to show the efficiency of the approach described in this presentation.