ORIGINAL ARTICLE Comparison of the anesthesia with thiopental sodium alone and their combination with Citrus aurantium L. (Rutaseae) essential oil in male rat

The aim of this study was to comparison of anesthesia with thiopental sodium alone and their combination with citrus aurantium L. essential oil in male rat. In this study 24 male Wistar rats (250 - 300 g) randomly assigned in 3 groups, Citrus aurantium L. essential oil alone (CA)(500mg/kg,i.p.), combination of Citrus aurantium L. essential oil - thiopental sodium (CAT)(500mg/kg and 50mg/kg, i.p., respectively)- and thiopental sodium alone (T)(5omg/kg,i.p.) (n=8). Heart and respiratory rate, induction and duration of surgical anesthesia (SA) walking times, withdrawal reflexes (pedal withdrawal, lip and tail pinches) were measured. Our results showed that all of groups induced SA. Thiopental had rapid induction time, short duration of SA and fast recovery. Instead, CAT group has late induction time and long duration of SA and late walking time. Heart rate and temperature were not changed significantly between groups (p>0.05). Respiratory rates was decreased in CA group compared to other groups (Between 0-40 min) (p<0.05). Lip, tail and pedal pinches not significant changed between groups (except in T group between35-55min), but T group weaker than other groups inhibited pain (p<0.05). In conclusion, anesthesia with CAT combination prefer to long time of surgeries and it seems GABAergic(GABAA)pathways involved CA anesthetic activity. But, further studies needed for determine this exact mechanism of action.