원저 : HMC05의 배양 Chinese Hamster Lung 세포를 이용한 염색체이상 시험

Objectives: We investigated genetic toxicity of HMCO5 in relation to chromosome aberration test on Cultured Chinese Hamster Lung (CHL) in the presence and absence of S-9 mix. Methods: Experimental groups were divided into two groups: with S-9mix (+S) or without S-9 mix (-S). -S group was also divided 2 series by treatment hours (6 hr: 6-S; or 24 hr; 24-S). Each group treated with vehicle only (complete culture medium), HMCO5 (1,250, 2,500, 5,000 ㎍/㎖), and cyclophosphamide monohydrate (CPA) and ethylmethanesulfonate (EMS), respectively. Results: HMC05 did not show any aberrant metaphase. However, there were significant (p<0.01) aberrant metaphases with CPA in S+ and with EMS in S-. Conclusions: These results indicate that HMC05 fomula does not show any toxicity in chromosome aberration test.