Real-time temperature-control micro sample room

The invention provides a real-time temperature-control micro sample room. The micro sample room comprises a temperature control device (A), a sample room body (B) and a temperature control instrument (C). The temperature control device (A) comprises a U-type supporting plate (1), a thermoelectric cooler (TEC) (2), a TEC metal heat-conduction piece (3), a thermocouple probe (4), a slide supporting plate (5) and a thermal insulation plate (6). The sample room body (B) comprises an upper cover slide (7), a sample room substrate (8) and a lower cover slide (9). The temperature control instrument (C) comprises a temperature control power supply, a feedback controller and a wire. The temperature of a sample can be controlled and adjusted in real time, the temperature control range is from 6 DEG C to 90 DEG C, the temperature response time is less than or equal to 3 min, and the temperature control accuracy ranges from minus 0.5 DEG C to 0.5 DEG C. No mechanical vibration is introduced to the sample room, no noise sources exist, the capacity is adjustable, the sample room can be full with at least 10 micro liters of solutions, the size is small and the sample room is especially suitable for being used in the situation that space of a microscope stage is very small due to a high-magnification-power objective lens.