Hydrogel Matrix Grafted Impedimetric Aptasensor for the Detection of Diclofenac

Driven by the growing concern about the release of the untreated emerging pollutants and the need for determining small amount of these pollutants present in the environment, novel biosensors dedicated to molecular recognition are developed. We have designed biosensors using a novel class of grafted polymers, surface-attached hydrogel thin films on conductive transducer as a biocompatible matrix for biomolecule immobilization. We showed that they can be dedicated to the molecular recognition of Diclofenac (DCL). The immobilization of the aptamer onto surface-attached hydrogel thin films by covalent attachment provides a biodegradable shelter, providing the aptamer excellent environments to preserve its active and functional structure while allowing the detection of DCL. The grafting of the aptamer is obtained using the formation of amide bonds via the activation of carboxylic acid groups of the poly (acrylic acid) (PAA) hydrogel thin film. For improved sensitivity and higher stability of the sensor, a hig...