Biodegradation of CO2, CH4 and volatile organic compounds (VOCs) in soil gas from the Vicano–Cimino hydrothermal system (central Italy)

Abstract We investigated the effect of microbial activity on the chemistry of hydrothermal fluids related to the Vicano–Cimino system, central Italy. The database included the composition and δ 13 C CO 2 and δ 13 C CH 4 values for soil gas from an area characterized by intense degassing of fluids having a deep origin. The δ 13 C CH 4 values along vertical profiles in the soil indicated that CH 4 was controlled by microbial oxidation occurring at shallow ( 2 was available. This was consistent with the vertical gradients of CH 4 , H 2 S and O 2 concentrations. The δ 13 C CO 2 values in soil gas, characterized by a composition similar to that of the hydrothermal fluids, were not significantly influenced by biodegradation. On the contrary, gas strongly affected by air contamination showed a significant δ 13 C CO 2 fractionation. Microbial activity caused strong consumption of hydrothermal alkanes, alkenes, cyclics and hydrogenated halocarbons, whereas benzene was recalcitrant. Oxygenated compounds from hydrocarbon degradation consisted of alcohols, with minor aldehydes, ketones and carboxylic acids. A predominance of alcohols at a high rate of degassing flux, corresponding to a short residence time of hydrothermal gas within the soil, indicated incomplete oxidation. N-bearing compounds were likely produced by humic substances in the soil and/or related to contamination by pesticides, whereas α-pinene traced air entering the soil. The study demonstrates that microbial communities in the soil play an important role for mitigating the release to the atmosphere of C-bearing gases, especially CH 4 , through diffuse soil degassing, a mechanism that in central Italy significantly contributes to the discharge of CO 2 -rich gas from deep sources.