Abstract 4725: The 3D nuclear telomere organization of Hodgkin-cells is different between patients entering rapid remission and patients with refractory or relapsing disease

Our recent study has utilized innovative 3D Q- FISH and three-dimensional (3D) imaging to define the 3D nuclear telomere organization in mono-nucleated Hodgkin (H) and multi-nucleated Reed-Sternberg (RS) cells of Hodgkin9s lymphoma (HL) derived cell lines and diagnostic patient biopsies (Leukemia. 2009). These characteristics were found in both, classical EBV negative and EBV-associated, LMP1 expressing HL (Lab Invest. 2010). However, it is still unknown whether the 3D telomere profile at diagnostic biopsy is different in patients entering rapid remission after initiation of standard chemotherapy (ABVD) compared to patients with refractory or relapsing disease. In this study, we investigated by 3D telomere Q-FISH diagnostic biopsies of HL patients entering rapid complete remission and diagnostic biopsies from patients with refractory or relapsing disease. 8 diagnostic biopsies of 8 patients entering rapid remission (after 1-4 cycles of ABVD) and 8 diagnostic biopsies of 5 patients including 2 with primary refractory disease (progressing after 4-8 cycles of ABVD) and 3 cases relapsing 1-3 years after late remission (post 6 -8 cycles of ABVD) were analyzed the 3D telomeric structure by 3D Q-FISH and TeloView software (Cytometry A. 2005). We found that RS-cells of all patients from both groups showed significant increase in very short telomeres when compared to the mononuclear precursor H-cells (p Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 4725. doi:10.1158/1538-7445.AM2011-4725