PARAFAC TREATMENT IN 3D -FQ OF DOM-(M) COMPLEXATION

The DOM-Metal complexation propriety is accessible by fluorescence quenching (FQ). FQ spectrum can determine the fluorescent DOM composition by parallel factor analysis (PARAFAC) with excitation-emission matrix (EEM). [Cu ] titration onto a river water sample from Bresil: [C] =5mg/L, initial [Cu ]=1.68 nM , pH=4.5, [Cu ]=10 M~10 M is done with 50 Excitation and Emission Matrix (EEM) analyzed by PARAFAC, which has extracted 2 fluorescent components from all EEM: C1 (λex=235nm/λem=420-425nm) and C2 (λex=250-260nm and 345-355nm/λem=470-480nm) of which corresponding interatures were obtained by this new approach. It is confirmed that with PARAFAC FQ modification values fluorophores are better distinguished from the whole matrix.