Combined Effects of pH and Borohydride Reduction on Optical Properties of Humic Substances (HS): A Comparison of Optical Models

The combined effects of pH and borohydride reduction on the optical properties of a series of humic substances and a lignin model were examined to probe the molecular moieties and interactions that give rise to the observed optical properties of these materials. Increasing the pH from 2 to 12 produced significantly enhanced absorption across the spectra of all samples, with distinct spectral responses observed over pH ranges attributable to the deprotonation of carboxylic acids and phenols. Borohydride reduction substantially attenuated the broadband absorption enhancements with pH, clearly indicating that the loss of absorption due to ketone/aldehyde reduction is coupled with the pH-dependent increase in absorption due to deprotonation of carboxylic acids and phenols. These results cannot be easily explained by a superposition of the spectra of independently absorbing chromophores (superposition model) but are readily interpretable within a charge transfer (CT) model. Changes of fluorescence emission wit...