Sub-cellular network analysis of ryanodine receptor positioning in control and phosphorylated states

Type 2 ryanodine receptors (RyR2) are large (mass=2.2 MegaDalton) proteins expressed in heart cells that mediate the release of calcium ions (Ca 2+ ) that, in turn, modulate contraction of the heart. In this work, we analyze the sub-cellular spatial distribution of RyR2 using data from superresolution microscopy, an imaging technique that allows highly accurate positioning ( 95% at proximity thresholds 200 and 250 nm). Our findings may help in better understanding Ca 2+ signaling during contraction and give insight into the changes that underlie its regulation.