Determination of cholesteryl palmitate in Oviductus Ranae by HPLC

Objective: To develop a new method for the determination of cholesteryl palmitate in Oviductus Ranae. Method: A HPLC method was set up, using Zorbax Silica column and cyclohexane-diethyl ether (40:1) as mobile phase with a flow rate of 1.0 mL·min-1, and the UV detection wavelength was 203 nm. Result: The calibration curve was linear over the range of 0.60-8.92 μg( r = 0.999 7) , the average recovery of the method was 98.4% . RSD 1.8% ( n = 6) . Conclusion: The results showed that method was reliable and accurate.