INVESTIGATION OF IN-VITRO ANTI-OXIDANT, ANTI-INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITY OF AERIAL PARTS OF SECURINEGA LEUCOPYRUS (WILLD.) MUELL

Securinega leucopyrus (willd.) Muell. (Euphorbiaceae) is a thorny, large shrub or small tree. In Ayurveda it has a lot of potential medicinal uses. The decoction of leaves are used to dress the cancerous wounds. The juice or paste of the leaves along with tobacco used to destroy worms in sores. Its leaf decoction useful externally in the treatment of piles. The present study is designed to carry out Anti-oxidant, Anti-inflammatory, Anti arthritic activity by Invitro methods. The Securinega leucopyrus was authenticated and the anatomical features were done which shows calcium oxalate crystals, fibres, glandular trichomes, sclereids, vascular bundles (secondary xylem and phloem). The microscopical evaluation also performed by determination of stomatal index and vein islet number. The In-vitro anti-oxidant activity revealed that the Alcoholic extract showed more activity as compared to Chloroform, Ethyl acetate, n-Hexane. In DPPH (1,1-diphenyl-2-picrylhydrazyl) radical method Alcoholic extract showed more activity and in nitric oxide scavenging method Hydro alcoholic, Alcoholic extracts showed more activity. Chloroform extract showed more activity in hydroxy radical method. In Total anti-oxidant method Alcoholic extract showed more activity. The Anti-inflammatory activity also performed by Human Red Blood Corpuscle (HRBC) Membrane Stabilization Method. The results were tabulated and the values are represented in the figure. In this Ethyl acetate extract showed more activity. The Anti-arthritic activity is performed by inhibition of protein denaturation method. And the results are given in table and values are represented in graph. This shows Alcoholic extract gave more activity. The above results shows that the presence of phytoconstituents like Glycosides, Flavanoids, Alkaloids, steroids and Tannins may be responsible for Anti-oxidant, Anti-inflammatory, Ant arthritic activities.