Early maternal separation promotes apoptosis in dentate gyrus and alters neurological behaviors in adolescent rats.

: Adverse early-life experience such as maternal separation (MS) affects the behavior of adult, and may also aggravate the outcome of neurological insults. In this study, we aimed to investigate the effects of early MS on hippocampus-related behaviors, and to assess the mechanisms. Newborn rats were randomly divided into normal control and MS groups. Our data showed that MS (P3-P21) impaired learning ability as well as memory retrieval, and caused depression-like activity, but decreased anxiety-like activity. Glutamate receptor 1 (GluR1) expression in the dentate gyrus (DG) region was significantly reduced in the adults (P60). Mechanically, MS promoted apoptosis, and reduced protein kinase B (AKT) phosphorylation in the DG region in the early phase (P21). By contrast, MS did not affect ERK phosphorylation. Our data implicate that the inactivation of AKT pathway and apoptosis of DG cells might contribute to MS-induced behavioral changes. This study would provide useful evidence implicating the pathological changes for MS.