Enzyme Replacement Therapy in the Pancreatic Duct Ligated Swine

SummaryPancreatic duct ligation (PDL) in swine reduced absorption of dietary fat and protein to less than 50%. Supplementation of the diet with pancreatin 4X NF at 0.1, 0.5 and 1.0% resulted in a significant improvement in fat absorption which approached normal on the highest level of pancreatin. Protein absorption was also improved but remained well below that observed in normal swine.Intestinal levels of trypsin, chymotrypsin, and lipase were elevated in the PDL pig for up to 2 hr following oral administration of 4 g pancreatin 4X NF. However, only in the case of lipase did the levels approach those found in the normal swine intestine.These studies also point out that the PDL may have applicability as a model for investigating pancreatin enzyme replacement therapy.