Insight into Polycation Chain Length Affecting Transfection Efficiency by O- Methyl-Free N,N,N -Trimethyl Chitosans as Gene Carriers

Purpose The structure–function relationship and mechanism of polycations as gene carriers have attracted considerable research interest in recent years. The present study was to investigate the relationship between polycation chain length and transfection efficiency (RCL-TE), and the corresponding mechanism by O-methyl-free N,N,N-trimethyl chitosans (TMCs) as gene carriers.