NUTRACEUTICAL EFFECTS OF MORINGA OLEIFERA, LEAVES, SEED AND EXTRACT ON SIDE EFFECTS OF ISOPROTERENOL INDUCED MYOCARDIAL DAMAGE IN MALE RATS

Abstract The present study evaluated preventive effect of Moringa oleifera leaves, seed powder and water extract of the isoproterenol (ISP)-induced myocardial infarction. Forty adult albino male rats Sprague –Dawley strain weighing 145 ±6 g were classified into five groups,(8 rats each) the first group was fed on basal diet only control negative(-ve) group and four rat groups which injected ISP (85 mg/kg, s.c.) at an interval of 24 hours. tow day1 and 2 to induce myocardial infarction .and reclassified into non-treated control positive (+ve) group was fed on basal diet only, and treated groups which were Moringa oleifera leaves, ,seed powder (500 mg/kg b.w) and Moringa oleifera water extract (300mg/kg b.w) in 10 ml of normal saline daily by stomach tube, for 60 days The results revealed that, the control (+ve) group showed a significant decrease in weight gain; food intake ,feed efficiency ratio(FER),protein efficiency ratio(PER),serum high density lipoprotein cholesterol (HDLc), superoxide dismutase( SOD) ,catalase (CAT) and glutathione-S-transferase (GST) but a significant increase in serum cholesterol (CHO), triglyceride(TG), low density lipoprotein cholesterol (LDLc), very low density lipoprotein cholesterol (VLDLc), Atherogenic indices CHO/HDL ,amino transferase, (AST,ALT), alkaline phosphates (ALP),Lactatet dehydrogenase(LDH) in heart tissue Lactatet dehydrogenase(LDH),Nitric oxide(NO)and Xanthine oxidase (XO) Compared with control (-ve) group. The Moringa oleifera leaves, seed powder and water extract rat groups showed a significant increase in weight gain; food intake , (FER), (PER),serum (HDLc) ,( SOD) , (CAT) and (GST).While showing a significant decrease in serum CHO, TG, (LDLc), (VLDLc), CHO/HDL, AST, ALT, ALP, LDH, and Malondialdehyde (MDA) in heart tissue LDH, NO and XO Compared with control (+ ve) group Histopathological examination revealed that, heart of myocardial infarction rat treated with Moringa oleifera leaves, ,seed powder and Moringa oleifera water extract showed apparent normal heart and non-histopathological changes These biochemical changes were accompanied with histopathological alterations.It can be concluded that the administration of Moringa oleifera leaves, seed and water extract can lower the side effects of isoproterenol induced myocardial infarction rats and reducing the risk factors for cardiovascular disease such as hyperlipidemia ,leakage of cardiac markers and healthy condition  Key words: Moringa oleifera leaves, seed extract , isoproterenol (ISP), rats