State discrimination method of cell mass, an image processing program and an image processing apparatus using this method, and manufacturing method of the cell mass

Providing means capable determine the state of the multi-layered cell mass from two of the observation image at a predetermined time. Image processing program (GP) is a reference to step (S10) of acquiring a first image and a second image photographed at a predetermined time by the imaging device, the luminance distribution of the local region of the first image, the second performing block matching in the vicinity including the corresponding position of the image, the most matching high area representative degree of approximation degrees of approximation, and the step (S40) of calculating a representative approximation of the cell mass each section by sequentially moving the local area , and a step (S50) for outputting the multi-layering information corresponding to the calculated representative similarity, first, outputs the multi-layering information that can determine the status of the multi-layered cell mass from the second image configured to.