The effect of vitamin E and plant extract mixture composed of carvacrol, cinnamaldehyde and capsaicin on oxidative stress induced by high PUFA load in young pigs

The objective of our study was to determine the antioxidative potential of a plant extract (PE) mixture composed of carvacrol,capsicum oleoresin and cinnamaldehyde against high n-3 polyunsaturated fatty acid (PUFA)-induced oxidative stress in youngpigs. Thirty-two weaned castrated male crossbred pigs (BW 10.9kg;n532) were randomly assigned to four dietary treatments(n58). The negative control diet (Cont) contained 17.2% energy from fat. Oxidative stress was induced in three of the fourexperimental groups with the inclusion of n-3 PUFA rich linseed oil. Linseed oil substituted wheat starch in the diet to elevatethe amount of energy from fat to 34.1%. One of these diets served as a positive control (Oil), one was additionallysupplemented with 271.2mg/kg of PE mixture and one with 90.4mg/kg a-tocopheryl acetate (Vit E). After 14 days of treatment,blood and urine were collected for the determination of lipid peroxidation and DNA damage. Lipid peroxidation was studied byplasma malondialdehyde (MDA) concentrations, 24h urinary MDA and F