Expression of cytokines, TNF-α and IL-1α, in MAM acetate and 1-hydroxyanthraquinone-induced colon carcinogenesis of rats

The expression of cytokines, TNF-α and IL-1α, was examined by means of a reverse transcription followed by PCR (RT- PCR) in rat colon carcinogenesis. Forty male F344 rats were used and divided into four groups. At the start of the experiment, 20 rats were treated with methylazoxymethanol (MAM) acetate (25 mg/kg body wt, one time, i.p.) and divided into two groups; group 1 was exposed to 1% 1-hydroxyanthraquinone (1-HAQ) and group 2 was fed a basal diet during the experiment (40 weeks). Other rats were also divided into two groups; group 3 was exposed to 1% 1-HAQ as group 1, and group 4 was used as control. Tumor incidence (100%) and multiplicity (5.00±2.05) in group 1 were significantly greater than those in group 2 (20% and 0.2±0.42) and group 3 (10% and 0.10±0.32) (P<0.01 and P<0.01 respectively)