Detection of survival time of cytokine-induced killer cells in vivo by specific DNA probes

Objective To observe the survival time of cytokine-induced killer cells (CIK) in vivo in gender-mismatched female recipients by using fluorescence in situ hybridization (FISH).Methods Mononuclear cells of male donors were collected with cell collection device,and induced into CIK cells by several kinds of cytokines in vitro,and then were infused to the female recipients.Several months after CIK treatment,the expression of Y chromosome in lymphocytes of the female recipients was detected by using Y chromosome specific DNA probes.Results CIK cells were highly expressed after 14 days,and CD3 +CD56 + was 55.4％ by flow cytometry.CSP X probe was located at Xp11.1-q11.1,and the hybridization signal was green; CSP Y probe was located at Yp11.1-q11.1,and the hybridization signal was red.Female cell chromosome was XX,showing two green fluorescent signals; Male cell chromosome was XY,showing one green fluorescent signal and one red fluorescent signal.Y chromosome was expressed in the lymphocytes of the female recipients,and the expression rate was 0.5％ to 3.0％.Conclusion The survival time of CIK cells in vivo can be determined by using Y chromosome specific DNA probes. Key words: Fluorescence in situ hybridization;  Cytokine-induced killer cells;  Survival time