Sorcin表达受抑乳腺癌多药耐药细胞株MCF-7/A02对化疗药物敏感性的观察

Objective To study the effect of soluble resistance-related calcium-binding protein gene (Sorcin) on multidrug resistance (MDR) in MCF-7/A02 cells, and explore the correlative mechanisms. Methods MCF-7/A02 cells were divided into observe group (transfected with Sorcin gene siRNA) and control group (non-transfection). The expression of Sorcin and proteins of Bcl-2, Bax were measured by western blot after siRNA transfection in MCF-7/A02 cells. The sensitivity of cells to ADR, VP-16, HHT and VCR were measured by MTT assays. The accumulation of cytotoxic agents in cells and the apoptotic rate to VP-16 after siRNA transfection were detect by FACS. Results The ADR, VP-16, HHT, VCR IC50 of the observe group were (19.92±1.29), (31.47±2.36), (4.19±0.27), (2.96±0.23) µg/ml, control group were (111.57±5.60), (144.77±20.43), (24.30±0.43), (5.89±0.28) µg/ml, all P＜0.01. Apoptosis rate of the treatment group was 9.15%±0.25%, control group was 4.1%±0.12%, P＜0.01. The cells MFI of observe group was 6.34±0.86, control group was 5.42±0.73, P＞0.05. Bcl-2/GAPDH, Bax/GAPDH light density ratio of the treatment group was 0.59±0.03 and l.80±0.03, control group was 0.69±0.20 and l.94±0.04, all P＜0.05. Conclusion The reduction of the expression of Sorcin leads to partly reversion of MDR phenotype in MCF-7/A02 cells, which is associated with the changes of Bcl-2 and Bax expression.