Simultaneous Determination of Kirenol and Darutigenol in Herba Siegesbeckiae by RP-HPLC

OBJECTIVE To establish a RP-HPLC method for simultaneous determination of kirenol and darutigenol in Herba Siegesbeckiae.METHODS The separation was performed on a Kromasi1 C18 column(4.6 mm×200 mm,5 μm).Acetonitrile-water was used as the mobile phase with gradient elution(0-6 min,29.5% acetonitrile,6-10 min,29.5%-43.5% acetonitrile).The flow rate was 1.0 mL·min-1.The column temperature was 30 ℃ and the UV detection wavelength was 215 nm.RESULTS The calibration curves of kirenol were in good linearity in the range of 0.015 1-0.302 4 mg·mL 1(r=0.999 8).The average recovery was 101.5%(RSD=1.7%,n=9).The calibration curves of darutigenol were in good linearity in the range of 0.002 6-0.052 mg·mL-1(r=0.999 7).The average recovery was 101.7%(RSD=2.0%,n=9).CONCLUSION The method is simple,accurate and sensitive with good reproducibility and could be used for the simultaneous determination of kirenol and darutigenol in Herba Siegesbeckiae.The contents of kirenol and darutigenol in Herba Siegesbeckiae from different places were compared by this method.