Recombinant escherichia coli and method for applying same to produce poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) by utilizing single carbon source

2011 
The invention discloses recombinant escherichia coli QW102PT and an application thereof in producing poly(3-hydroxybutyrate-3-hydroxyvalerate) (PHBV) by utilizing a single carbon source. The recombinant escherichia coli can be used for efficiently utilizing the single carbon source to synthesize the PHBV, and the mole fraction of 3HV (3-hydroxyvaleric acid) is improved from 0.45 percent to 17.5 percent compared with that of recombinant bacteria DH5 alpha (pBHR68), which has obvious significance to commercial production of the PHBV. According to the invention, the problems that the cost is overhigh as propionic acid is used as an auxiliary carbon source and control strategies during the production process are excessively complicated are solved, and a new concept of synthesizing the PHBV byutilizing a simple carbon source is created.
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