[Triptolide inhibits proliferation and induces apoptosis of imatinib resistant K562/G01 cells].

This study was aimed to explore the inhibitory effect of triptolide on proliferation and inducing apoptosis effect of K562/G01 cells and their possible mechanism.MTT assay was used to detect the effect of imatinib or triptolide alone and their combination on K562/G01 proliferation;the cell cycle,apoptosis rate,P-gp protein expression were detected by folw cytometry(FCM);the expression of P-gp was assessed by Western blot;the BCR/ABL gene expression was assayed by real time guantitative PCR.The results showed that triptolide could enhance the effect of imatinib on proliferation inhibition and apoptosis of K562/G01,arrested the cell cycle in G1 phase,down-regulated the expression of BCR/ABL gene and P-gp protein.It is concluded that triptolide induces K562/G01 cell proliferation inhibition and apoptosis,the mechanism may be related to cell cycle arrest,decrease of P-gp protein expression,inhibition of BCR/ABL gene expression.