Progress on the determination of liposome encapsulated volume

Objective To review the methods on the determination of liposome encapsulated volume.Methods The review was summarized on the basis of 57 published literatures.Results Based on different principles,the determination of liposome encapsulated volume was divided into two types:theoretical model and experimental determination.Experimental determination included solute encapsulation method,electron spin resonance(ESR) method,conductivity and nuclear magnetic resonance(NMR) method.Conclusions The article provides an important reference for research and development of encapsulated water-soluble,nucleic acid and protein drugs in the aqueous phase of the liposome.