Analyzing trapped protein complexes by Virotrap and SFINX

Kevin Titeca,Emmy Van Quickelberghe,Noortje Samyn,Delphine De Sutter,Annick Verhee,Kris Gevaert,Jan Tavernier,Sven Eyckerman

Analyzing trapped protein complexes by Virotrap and SFINX

2017

Kevin Titeca
Emmy Van Quickelberghe
Noortje Samyn
Delphine De Sutter
Annick Verhee
Kris Gevaert
Jan Tavernier
Sven Eyckerman

This protocol enables the study of protein complexes by trapping them in virus-like particles and thereby preserving cellular integrity. The Virotrap protocol is complemented by SFINX, an online data analysis tool for interactomics studies.

Keywords:

Cell biology
Affinity chromatography
Molecular biology
Biology
Proteomics
Bioinformatics
Lysis
Plasma protein binding
Protein Interaction Map
Protein–protein interaction
Protein Interaction Networks
Protein multimerization

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